CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
ALK1; ALP; MPI; BLPI; HUSI; HUSI-I; WAP4; WFDC4; Antileukoproteinase; Seminal proteinase inhibitor; Mucus proteinase inhibitor; WAP four-disulfide core domain protein 4
- Product No.SCB312Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range1.37-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.64pg/mL.
- Sample TypeSerum, plasma and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 605
For more details, please contact local distributors!US$ 864
For more details, please contact local distributors! US$ 3888
For more details, please contact local distributors! US$ 7344
For more details, please contact local distributors! US$ 60480
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
This assay has high sensitivity and excellent specificity for detection of Secretory Leukocyte Peptidase Inhibitor (SLPI).
No significant cross-reactivity or interference between Secretory Leukocyte Peptidase Inhibitor (SLPI) and analogues was observed.
Recovery of the CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
Matrices listed below were spiked with certain level of recombinant Secretory Leukocyte Peptidase Inhibitor (SLPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Secretory Leukocyte Peptidase Inhibitor (SLPI) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-96 | 92 |
| EDTA plasma(n=5) | 78-90 | 85 |
| heparin plasma(n=5) | 93-105 | 99 |
Precision of the CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secretory Leukocyte Peptidase Inhibitor (SLPI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-99% | 91-98% | 88-105% | 93-101% |
| EDTA plasma(n=5) | 78-92% | 93-105% | 90-99% | 88-99% |
| heparin plasma(n=5) | 95-105% | 79-90% | 78-104% | 80-91% |
Stability of the CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle of the CLIA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)
The microplate provided in this kit has been pre-coated with an antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Secretory Leukocyte Peptidase Inhibitor (SLPI) level in the sample or standard.;
GIVEAWAYS
INCREMENT SERVICES
Single-component Reagents of Assay Kit
Lysis Buffer Specific for ELISA / CLIA
Quality Control of Kit
CLIA Kit Customized Service
Disease Model Customized Service
Serums Customized Service
TGFB1 Activation Reagent
Real Time PCR Experimental Service
Streptavidin
Fast blue Protein Stain solution
Single-component Reagents of FLIA Kit
Streptavidin-Agarose Beads