CLIA Kit for Sclerostin (SOST)

VBCH; Sclerosteosis

  • CLIA Kit for Sclerostin (SOST) Packages (Simulation)
  • CLIA Kit for Sclerostin (SOST) Packages (Simulation)
  • CLIA Kit for Sclerostin (SOST) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Sclerostin (SOST)

This assay has high sensitivity and excellent specificity for detection of Sclerostin (SOST).
No significant cross-reactivity or interference between Sclerostin (SOST) and analogues was observed.

Recovery of the CLIA Kit for Sclerostin (SOST)

Matrices listed below were spiked with certain level of recombinant Sclerostin (SOST) and the recovery rates were calculated by comparing the measured value to the expected amount of Sclerostin (SOST) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 94-105 97
heparin plasma(n=5) 86-94 90

Precision of the CLIA Kit for Sclerostin (SOST)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sclerostin (SOST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sclerostin (SOST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Sclerostin (SOST)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sclerostin (SOST) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 79-95% 83-104% 95-104%
EDTA plasma(n=5) 82-98% 78-88% 94-102% 91-105%
heparin plasma(n=5) 91-101% 93-105% 83-92% 99-105%

Stability of the CLIA Kit for Sclerostin (SOST)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Sclerostin (SOST)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Sclerostin (SOST)

The microplate provided in this kit has been pre-coated with an antibody specific to Sclerostin (SOST). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Sclerostin (SOST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Sclerostin (SOST) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPC864Mu01 Recombinant Sclerostin (SOST) Positive Control; Immunogen; SDS-PAGE; WB.
RPC864Mu02 Recombinant Sclerostin (SOST) Positive Control; Immunogen; SDS-PAGE; WB.
PAC864Mu01 Polyclonal Antibody to Sclerostin (SOST) WB; IHC; ICC; IP.
PAC864Mu02 Polyclonal Antibody to Sclerostin (SOST) WB; IHC; ICC; IP.
SEC864Mu ELISA Kit for Sclerostin (SOST) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC864Mu CLIA Kit for Sclerostin (SOST) Chemiluminescent immunoassay for Antigen Detection.
LMC864Mu Multiplex Assay Kit for Sclerostin (SOST) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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