CLIA Kit for Osteopontin (OPN)

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

  • CLIA Kit for Osteopontin (OPN) Packages (Simulation)
  • CLIA Kit for Osteopontin (OPN) Packages (Simulation)
  • CLIA Kit for Osteopontin (OPN) Results demonstration
  • SCA899Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Osteopontin (OPN)

This assay has high sensitivity and excellent specificity for detection of Osteopontin (OPN).
No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.

Recovery of the CLIA Kit for Osteopontin (OPN)

Matrices listed below were spiked with certain level of recombinant Osteopontin (OPN) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteopontin (OPN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-92 87
EDTA plasma(n=5) 90-101 97
heparin plasma(n=5) 80-104 101

Precision of the CLIA Kit for Osteopontin (OPN)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteopontin (OPN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Osteopontin (OPN)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteopontin (OPN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-89% 95-102% 99-105% 81-92%
EDTA plasma(n=5) 81-90% 81-101% 90-104% 94-101%
heparin plasma(n=5) 89-97% 94-101% 95-102% 90-97%

Stability of the CLIA Kit for Osteopontin (OPN)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Osteopontin (OPN)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Osteopontin (OPN)

The microplate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Osteopontin (OPN) level in the sample or standard.;

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