CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

NAP3; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein

  • CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Packages (Simulation)
  • CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Packages (Simulation)
  • CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

This assay has high sensitivity and excellent specificity for detection of Chemokine (C-X-C Motif) Ligand 1 (CXCL1).
No significant cross-reactivity or interference between Chemokine (C-X-C Motif) Ligand 1 (CXCL1) and analogues was observed.

Recovery of the CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

Matrices listed below were spiked with certain level of recombinant Chemokine (C-X-C Motif) Ligand 1 (CXCL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine (C-X-C Motif) Ligand 1 (CXCL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-103 99
EDTA plasma(n=5) 94-104 101
heparin plasma(n=5) 90-104 96

Precision of the CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine (C-X-C Motif) Ligand 1 (CXCL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-102% 79-101% 95-105% 96-105%
EDTA plasma(n=5) 93-103% 96-104% 94-103% 79-93%
heparin plasma(n=5) 79-98% 96-103% 79-91% 88-102%

Stability of the CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine (C-X-C Motif) Ligand 1 (CXCL1) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
EPA041Mu61 Eukaryotic Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA041Mu02 Recombinant Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA041Mu03 Recombinant Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA041Mu01 Recombinant Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA041Mu01 Polyclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1) WB; IHC; ICC; IP.
PAA041Mu02 Polyclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1) WB; IHC; ICC; IP.
PAA041Mu03 Polyclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1) WB; IHC; ICC; IP.
LAA041Mu71 Biotin-Linked Polyclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1) WB; IHC; ICC.
LAA041Mu81 FITC-Linked Polyclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1) WB; IHC; ICC; IF.
SEA041Mu ELISA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA041Mu Mini Samples ELISA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA041Mu CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Chemiluminescent immunoassay for Antigen Detection.
KSA041Mu01 ELISA Kit DIY Materials for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Main materials for "Do It(ELISA Kit) Yourself"
GUESTBOOK