CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8

  • CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Packages (Simulation)
  • CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Packages (Simulation)
  • CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Results demonstration
  • SCB286Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

This assay has high sensitivity and excellent specificity for detection of Milk Fat Globule EGF Factor 8 (MFGE8).
No significant cross-reactivity or interference between Milk Fat Globule EGF Factor 8 (MFGE8) and analogues was observed.

Recovery of the CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Matrices listed below were spiked with certain level of recombinant Milk Fat Globule EGF Factor 8 (MFGE8) and the recovery rates were calculated by comparing the measured value to the expected amount of Milk Fat Globule EGF Factor 8 (MFGE8) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-102 97
EDTA plasma(n=5) 85-102 97
heparin plasma(n=5) 91-101 96

Precision of the CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Milk Fat Globule EGF Factor 8 (MFGE8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-97% 92-99% 95-105% 87-102%
EDTA plasma(n=5) 98-105% 92-99% 95-102% 87-101%
heparin plasma(n=5) 81-96% 83-101% 94-103% 98-105%

Stability of the CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

The microplate provided in this kit has been pre-coated with an antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Milk Fat Globule EGF Factor 8 (MFGE8) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPB286Hu01 Recombinant Milk Fat Globule EGF Factor 8 (MFGE8) Positive Control; Immunogen; SDS-PAGE; WB.
PAB286Hu01 Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) WB; IHC; ICC; IP.
MAB286Hu22 Monoclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) WB; IHC; ICC; IP.
SEB286Hu ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB286Hu CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) Chemiluminescent immunoassay for Antigen Detection.
LMB286Hu Multiplex Assay Kit for Milk Fat Globule EGF Factor 8 (MFGE8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK