CLIA Kit for Luteinizing Hormone (LH)

ICSH; Lutropin; Interstitial Cell Stimulating Hormone

  • CLIA Kit for Luteinizing Hormone (LH) Packages (Simulation)
  • CLIA Kit for Luteinizing Hormone (LH) Packages (Simulation)
  • CLIA Kit for Luteinizing Hormone (LH) Results demonstration
  • CCA441Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Luteinizing Hormone (LH)

This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH).
No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.

Recovery of the CLIA Kit for Luteinizing Hormone (LH)

Matrices listed below were spiked with certain level of recombinant Luteinizing Hormone (LH) and the recovery rates were calculated by comparing the measured value to the expected amount of Luteinizing Hormone (LH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 79-98 82
heparin plasma(n=5) 98-105 102

Precision of the CLIA Kit for Luteinizing Hormone (LH)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Luteinizing Hormone (LH)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Luteinizing Hormone (LH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 95-103% 94-105% 91-99%
EDTA plasma(n=5) 80-105% 84-93% 85-94% 78-101%
heparin plasma(n=5) 96-103% 97-105% 90-98% 88-102%

Stability of the CLIA Kit for Luteinizing Hormone (LH)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Luteinizing Hormone (LH)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

Test principle of the CLIA Kit for Luteinizing Hormone (LH)

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Luteinizing Hormone (LH). A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Luteinizing Hormone (LH) level in the sample or standard.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
CEA441Mu ELISA Kit for Luteinizing Hormone (LH) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA441Mu Mini Samples ELISA Kit for Luteinizing Hormone (LH) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA441Mu CLIA Kit for Luteinizing Hormone (LH) Chemiluminescent immunoassay for Antigen Detection.
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