CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)

  • CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) Packages (Simulation)
  • CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) Packages (Simulation)
  • CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)

This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat Containing Protein 3C (LRRC3C).
No significant cross-reactivity or interference between Leucine Rich Repeat Containing Protein 3C (LRRC3C) and analogues was observed.

Precision of the CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)

The microplate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Leucine Rich Repeat Containing Protein 3C (LRRC3C) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPW764Hu01 Recombinant Leucine Rich Repeat Containing Protein 3C (LRRC3C) Positive Control; Immunogen; SDS-PAGE; WB.
PAW764Hu01 Polyclonal Antibody to Leucine Rich Repeat Containing Protein 3C (LRRC3C) WB; IHC; ICC; IP.
MAW764Hu21 Monoclonal Antibody to Leucine Rich Repeat Containing Protein 3C (LRRC3C) WB; IHC; ICC; IP.
SEW764Hu ELISA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) Enzyme-linked immunosorbent assay for Antigen Detection.
SCW764Hu CLIA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) Chemiluminescent immunoassay for Antigen Detection.
LMW764Hu Multiplex Assay Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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