CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

P19; IL23P19; SGRF

  • CLIA Kit for Interleukin 23 Subunit Alpha (IL23a) Packages (Simulation)
  • CLIA Kit for Interleukin 23 Subunit Alpha (IL23a) Packages (Simulation)
  • CLIA Kit for Interleukin 23 Subunit Alpha (IL23a) Results demonstration
  • SCA384Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

This assay has high sensitivity and excellent specificity for detection of Interleukin 23 Subunit Alpha (IL23a).
No significant cross-reactivity or interference between Interleukin 23 Subunit Alpha (IL23a) and analogues was observed.

Recovery of the CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

Matrices listed below were spiked with certain level of recombinant Interleukin 23 Subunit Alpha (IL23a) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 23 Subunit Alpha (IL23a) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-95 91
EDTA plasma(n=5) 91-105 95
heparin plasma(n=5) 90-97 93

Precision of the CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 23 Subunit Alpha (IL23a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 23 Subunit Alpha (IL23a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 23 Subunit Alpha (IL23a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 89-96% 92-99% 88-97%
EDTA plasma(n=5) 82-97% 98-105% 83-90% 95-104%
heparin plasma(n=5) 80-102% 79-89% 95-102% 82-92%

Stability of the CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Interleukin 23 Subunit Alpha (IL23a)

The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 23 Subunit Alpha (IL23a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 23 Subunit Alpha (IL23a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 23 Subunit Alpha (IL23a) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA384Mu01 Recombinant Interleukin 23 Subunit Alpha (IL23a) Positive Control; Immunogen; SDS-PAGE; WB.
RPA384Mu02 Recombinant Interleukin 23 Subunit Alpha (IL23a) Positive Control; Immunogen; SDS-PAGE; WB.
PAA384Mu02 Polyclonal Antibody to Interleukin 23 Subunit Alpha (IL23a) WB; IHC; ICC; IP.
PAA384Mu01 Polyclonal Antibody to Interleukin 23 Subunit Alpha (IL23a) WB; IHC; ICC; IP.
MEA384Mu Mini Samples ELISA Kit for Interleukin 23 Subunit Alpha (IL23a) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA384Mu ELISA Kit for Interleukin 23 Subunit Alpha (IL23a) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA384Mu CLIA Kit for Interleukin 23 Subunit Alpha (IL23a) Chemiluminescent immunoassay for Antigen Detection.
LMA384Mu Multiplex Assay Kit for Interleukin 23 Subunit Alpha (IL23a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK