CLIA Kit for Folic Acid (FA)

VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate

  • CLIA Kit for Folic Acid (FA) Packages (Simulation)
  • CLIA Kit for Folic Acid (FA) Packages (Simulation)
  • CLIA Kit for Folic Acid (FA) Results demonstration
  • CCA610Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Folic Acid (FA)

This assay has high sensitivity and excellent specificity for detection of Folic Acid (FA).
No significant cross-reactivity or interference between Folic Acid (FA) and analogues was observed.

Recovery of the CLIA Kit for Folic Acid (FA)

Matrices listed below were spiked with certain level of Folic Acid (FA) and the recovery rates were calculated by comparing the measured value to the expected amount of Folic Acid (FA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 78-92 86
heparin plasma(n=5) 96-103 101

Precision of the CLIA Kit for Folic Acid (FA)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folic Acid (FA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folic Acid (FA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Folic Acid (FA)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Folic Acid (FA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-93% 80-98% 86-98% 78-101%
EDTA plasma(n=5) 85-102% 95-103% 94-105% 91-99%
heparin plasma(n=5) 80-101% 97-104% 88-99% 79-90%

Stability of the CLIA Kit for Folic Acid (FA)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Folic Acid (FA)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

Test principle of the CLIA Kit for Folic Acid (FA)

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Folic Acid (FA). A competitive inhibition reaction is launched between biotin labeled Folic Acid (FA) and unlabeled Folic Acid (FA) (Standards or samples) with the pre-coated antibody specific to Folic Acid (FA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Folic Acid (FA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Folic Acid (FA) level in the sample or standard.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
CPA610Ge11 BSA Conjugated Folic Acid (FA) Immunogen; SDS-PAGE; WB.
CPA610Ge21 OVA Conjugated Folic Acid (FA) Immunogen; SDS-PAGE; WB.
CPA610Ge41 HSA Conjugated Folic Acid (FA) Immunogen; SDS-PAGE; WB.
PAA610Ge01 Polyclonal Antibody to Folic Acid (FA) WB; IHC; ICC; IP.
LAA610Ge81 FITC-Linked Polyclonal Antibody to Folic Acid (FA) WB; IHC; ICC; IF.
LAA610Ge71 Biotin-Linked Polyclonal Antibody to Folic Acid (FA) WB; IHC; ICC.
CEA610Ge ELISA Kit for Folic Acid (FA) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA610Ge CLIA Kit for Folic Acid (FA) Chemiluminescent immunoassay for Antigen Detection.
KSA610Ge11 ELISA Kit DIY Materials for Folic Acid (FA) Main materials for "Do It (ELISA Kit) Yourself".
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