CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

ZFGF5

  • CLIA Kit for Fibroblast Growth Factor 18 (FGF18) Packages (Simulation)
  • CLIA Kit for Fibroblast Growth Factor 18 (FGF18) Packages (Simulation)
  • CLIA Kit for Fibroblast Growth Factor 18 (FGF18) Results demonstration
  • SCC907Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 18 (FGF18).
No significant cross-reactivity or interference between Fibroblast Growth Factor 18 (FGF18) and analogues was observed.

Recovery of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 18 (FGF18) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 18 (FGF18) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-104 101
EDTA plasma(n=5) 86-101 89
heparin plasma(n=5) 79-92 88

Precision of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 18 (FGF18) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 83-97% 97-104% 78-101%
EDTA plasma(n=5) 79-98% 99-105% 80-95% 96-105%
heparin plasma(n=5) 86-94% 95-102% 88-102% 96-104%

Stability of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 18 (FGF18). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 18 (FGF18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 18 (FGF18) level in the sample or standard.;

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPC907Mu01 Recombinant Fibroblast Growth Factor 18 (FGF18) Positive Control; Immunogen; SDS-PAGE; WB.
RPC907Mu02 Recombinant Fibroblast Growth Factor 18 (FGF18) Positive Control; Immunogen; SDS-PAGE; WB.
PAC907Mu01 Polyclonal Antibody to Fibroblast Growth Factor 18 (FGF18) WB; IHC; ICC; IP.
PAC907Mu02 Polyclonal Antibody to Fibroblast Growth Factor 18 (FGF18) WB; IHC; ICC; IP.
MEC907Mu Mini Samples ELISA Kit for Fibroblast Growth Factor 18 (FGF18) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC907Mu ELISA Kit for Fibroblast Growth Factor 18 (FGF18) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC907Mu CLIA Kit for Fibroblast Growth Factor 18 (FGF18) Chemiluminescent immunoassay for Antigen Detection.
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