CLIA Kit for Coagulation Factor VIII (F8)

FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein

  • CLIA Kit for Coagulation Factor VIII (F8) Packages (Simulation)
  • CLIA Kit for Coagulation Factor VIII (F8) Packages (Simulation)
  • CLIA Kit for Coagulation Factor VIII (F8) Results demonstration
  • SCB878Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Coagulation Factor VIII (F8)

This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VIII (F8).
No significant cross-reactivity or interference between Coagulation Factor VIII (F8) and analogues was observed.

Recovery of the CLIA Kit for Coagulation Factor VIII (F8)

Matrices listed below were spiked with certain level of recombinant Coagulation Factor VIII (F8) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor VIII (F8) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 79-101 96

Precision of the CLIA Kit for Coagulation Factor VIII (F8)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Coagulation Factor VIII (F8)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor VIII (F8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 81-104% 78-89% 95-105% 96-103%

Stability of the CLIA Kit for Coagulation Factor VIII (F8)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Coagulation Factor VIII (F8)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Coagulation Factor VIII (F8)

The microplate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Coagulation Factor VIII (F8) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPB878Hu02 Recombinant Coagulation Factor VIII (F8) Positive Control; Immunogen; SDS-PAGE; WB.
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LAB878Hu73 Biotin-Linked Polyclonal Antibody to Coagulation Factor VIII (F8) WB; IHC; ICC.
MAB878Hu22 Monoclonal Antibody to Coagulation Factor VIII (F8) WB; IHC; ICC; IP.
SEB878Hu ELISA Kit for Coagulation Factor VIII (F8) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB878Hu CLIA Kit for Coagulation Factor VIII (F8) Chemiluminescent immunoassay for Antigen Detection.
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