CLIA Kit for C4 Binding Protein Beta (C4BPb)

C4BP-B; C4-BP; Complement Component 4 Binding Protein, Beta

  • CLIA Kit for C4 Binding Protein Beta (C4BPb) Packages (Simulation)
  • CLIA Kit for C4 Binding Protein Beta (C4BPb) Packages (Simulation)
  • CLIA Kit for C4 Binding Protein Beta (C4BPb) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for C4 Binding Protein Beta (C4BPb)

This assay has high sensitivity and excellent specificity for detection of C4 Binding Protein Beta (C4BPb).
No significant cross-reactivity or interference between C4 Binding Protein Beta (C4BPb) and analogues was observed.

Recovery of the CLIA Kit for C4 Binding Protein Beta (C4BPb)

Matrices listed below were spiked with certain level of recombinant C4 Binding Protein Beta (C4BPb) and the recovery rates were calculated by comparing the measured value to the expected amount of C4 Binding Protein Beta (C4BPb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-101 89
EDTA plasma(n=5) 84-101 90
heparin plasma(n=5) 79-96 79

Precision of the CLIA Kit for C4 Binding Protein Beta (C4BPb)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C4 Binding Protein Beta (C4BPb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C4 Binding Protein Beta (C4BPb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for C4 Binding Protein Beta (C4BPb)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4 Binding Protein Beta (C4BPb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 80-101% 88-102% 80-97%
EDTA plasma(n=5) 93-101% 91-99% 83-95% 80-91%
heparin plasma(n=5) 78-98% 94-105% 79-90% 97-105%

Stability of the CLIA Kit for C4 Binding Protein Beta (C4BPb)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for C4 Binding Protein Beta (C4BPb)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for C4 Binding Protein Beta (C4BPb)

The microplate provided in this kit has been pre-coated with an antibody specific to C4 Binding Protein Beta (C4BPb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C4 Binding Protein Beta (C4BPb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the C4 Binding Protein Beta (C4BPb) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA761Hu01 Recombinant C4 Binding Protein Beta (C4BPb) Positive Control; Immunogen; SDS-PAGE; WB.
PAA761Hu01 Polyclonal Antibody to C4 Binding Protein Beta (C4BPb) WB; IHC; ICC; IP.
LAA761Hu81 FITC-Linked Polyclonal Antibody to C4 Binding Protein Beta (C4BPb) WB; IHC; ICC; IF.
LAA761Hu71 Biotin-Linked Polyclonal Antibody to C4 Binding Protein Beta (C4BPb) WB; IHC; ICC.
MAA761Hu21 Monoclonal Antibody to C4 Binding Protein Beta (C4BPb) WB; IHC; ICC; IP.
SEA761Hu ELISA Kit for C4 Binding Protein Beta (C4BPb) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA761Hu CLIA Kit for C4 Binding Protein Beta (C4BPb) Chemiluminescent immunoassay for Antigen Detection.
GUESTBOOK