CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

BMP2A; BMP-2A; Hemochromatosis Modifier

  • CLIA Kit for Bone Morphogenetic Protein 2 (BMP2) Packages (Simulation)
  • CLIA Kit for Bone Morphogenetic Protein 2 (BMP2) Packages (Simulation)
  • CLIA Kit for Bone Morphogenetic Protein 2 (BMP2) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2).
No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.

Recovery of the CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

Matrices listed below were spiked with certain level of recombinant Bone Morphogenetic Protein 2 (BMP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Bone Morphogenetic Protein 2 (BMP2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-98 83
EDTA plasma(n=5) 84-101 97
heparin plasma(n=5) 78-104 94

Precision of the CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bone Morphogenetic Protein 2 (BMP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-102% 94-101% 89-97% 82-103%
EDTA plasma(n=5) 87-95% 79-99% 91-104% 93-103%
heparin plasma(n=5) 82-101% 78-90% 89-96% 80-98%

Stability of the CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)

The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 2 (BMP2) level in the sample or standard.;

Related products

Catalog No. Organism species: Sus scrofa; Porcine (Pig) Applications (RESEARCH USE ONLY!)
RPA013Po01 Recombinant Bone Morphogenetic Protein 2 (BMP2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA013Po02 Recombinant Bone Morphogenetic Protein 2 (BMP2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA013Po01 Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2) WB; IHC; ICC; IP.
PAA013Po02 Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2) WB; IHC; ICC; IP.
LAA013Po71 Biotin-Linked Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2) WB; IHC; ICC.
SEA013Po ELISA Kit for Bone Morphogenetic Protein 2 (BMP2) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA013Po CLIA Kit for Bone Morphogenetic Protein 2 (BMP2) Chemiluminescent immunoassay for Antigen Detection.
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