Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)
- Product No.PAE698Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPE698Hu01-Recombinant Sphingosine Kinase 1 (SPHK1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 105
For more details, please contact local distributors!US$ 245
For more details, please contact local distributors! US$ 350
For more details, please contact local distributors! US$ 875
For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human SPHK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human SPHK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant SPHK1, Human.
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Figure. Western Blot; Lane1: Human Raji Tissue; Lane2: Mouse Lung Tissue.
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Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human SPHK1 Polyclonal Antibody (Catalog # PAE698Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)
The antibody is a rabbit polyclonal antibody raised against SPHK1. It has been selected for its ability to recognize SPHK1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPE698Hu01 | Recombinant Sphingosine Kinase 1 (SPHK1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE698Hu01 | Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) | WB; IHC |
| FAE698Hu01 | Anti-Sphingosine Kinase 1 (SPHK1) Polyclonal Antibody | Flow cytometry. |
| MAE698Hu21 | Monoclonal Antibody to Sphingosine Kinase 1 (SPHK1) | WB; IHC; ICC; IP. |
| SEE698Hu | ELISA Kit for Sphingosine Kinase 1 (SPHK1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME698Hu | Multiplex Assay Kit for Sphingosine Kinase 1 (SPHK1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |