Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle

  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Packages (Simulation)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Packages (Simulation)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human P
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Skin cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Prostate cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA588Hu01.jpg Figure. Western Blot; Sample: Recombinant PKM2, Human.
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: PKM2 knockout MCF7 cell lysate;
    Predicted MW: 56,58kd
    Observed MW: 60kd
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) K562 human chronic myelogenous leukemia cell line was stained with rabbit Anti-human PKM2 Polyclonal Antibody (Catalog # PAA588Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

The antibody is a rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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