Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)
PHP14; Phosphohistidine Phosphatase 14kDa; Sex-Regulated Protein Janus-a; Protein janus-A homolog
- Product No.PAH413Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPH413Hu01-Recombinant Phosphohistidine Phosphatase 1 (PHPT1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 105
For more details, please contact local distributors!US$ 245
For more details, please contact local distributors! US$ 350
For more details, please contact local distributors! US$ 875
For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Brain Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PHPT1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 20µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant PHPT1, Human.
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Western Blot; Sample: MCF7 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: PHPT1 knockout 293T cell lysate;
Predicted MW: 14kd
Observed MW: 15kd
Primary Ab: 1µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: A549 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: 293T cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PHPT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)
The antibody is a rabbit polyclonal antibody raised against PHPT1. It has been selected for its ability to recognize PHPT1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPH413Hu01 | Recombinant Phosphohistidine Phosphatase 1 (PHPT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH413Hu01 | Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) | WB,IHC,ICC/IF |
| LAH413Hu71 | Biotin-Linked Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) | WB; IHC; ICC. |
| LAH413Hu81 | FITC-Linked Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) | WB; IHC; ICC; IF. |