Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
PFK-C; PFKF; ATP-dependent 6-phosphofructokinase, platelet type; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; Phosphohexokinase
- Product No.PAB745Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB745Hu01-Recombinant Phosphofructokinase, Platelet (PFKP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 100
For more details, please contact local distributors!US$ 232
For more details, please contact local distributors! US$ 332
For more details, please contact local distributors! US$ 830
For more details, please contact local distributors! US$ 3320
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Cardiac Muscle Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Spleen Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: A549 cell lysate; Lane4: Jurkat cell lysate Primary Ab: 0.3µg/ml Rabbit Anti-Human PFKP Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
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Western Blot; Sample: Lane1: A549 cell lysate; Lane2: Porcine Heart lysate; Lane3: Jurkat cell lysate; Lane4: Porcine Skeletal muscle lysate
Primary Ab: 3µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: PFKP knockout A549 cell lysate;
Predicted MW: 85kd
Observed MW: 85kd
Primary Ab: 3µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PFKP Polyclonal Antibody (Catalog PAB745Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
The antibody is a rabbit polyclonal antibody raised against PFKP. It has been selected for its ability to recognize PFKP in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB745Hu01 | Recombinant Phosphofructokinase, Platelet (PFKP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB745Hu01 | Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP) | WB,IHC |
| MAB745Hu22 | Monoclonal Antibody to Phosphofructokinase, Platelet (PFKP) | WB; IHC; ICC; IP. |
| SEB745Hu | ELISA Kit for Phosphofructokinase, Platelet (PFKP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB745Hu | Multiplex Assay Kit for Phosphofructokinase, Platelet (PFKP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |