Polyclonal Antibody to Nucleophosmin 1 (NPM1)
B23; NPM; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38
- Product No.PAC664Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC664Mu02-Recombinant Nucleophosmin 1 (NPM1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 108
For more details, please contact local distributors!US$ 252
For more details, please contact local distributors! US$ 360
For more details, please contact local distributors! US$ 900
For more details, please contact local distributors! US$ 3600
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Adipose tissue Tissue; Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Stomach Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Small intestine Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Pancreas Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Uterus Tissue
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 Cells;
Primary Ab: 10µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 7µg/ml Rabbit Anti-Mouse NPM1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant NPM, Mouse.
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Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Jurkat cell lysate; Lane3: K562 cell lysate
Primary Ab: 1µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: NPM knockout Jurkat cell lysate;
Predicted MW: 33kd
Observed MW: 38kd
Primary Ab: 1µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
The antibody is a rabbit polyclonal antibody raised against NPM1. It has been selected for its ability to recognize NPM1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Nucleophosmin 1 (NPM1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPC664Mu01 | Recombinant Nucleophosmin 1 (NPM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC664Mu02 | Recombinant Nucleophosmin 1 (NPM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC664Mu02 | Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB,IHC,ICC/IF |
| PAC664Mu01 | Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC; IP. |
| LAC664Mu71 | Biotin-Linked Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC. |