Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA)

MHCDRa; HLADRa; Major Histocompatibility Complex Class II DR Alpha

  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Packages (Simulation)
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Packages (Simulation)
  • PAC166Hu01.jpg Figure. Western Blot; Sample: Recombinant MHCDRa, Human.
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Knockout Varification:
    Lane 1: Wild-type Jurkat cell lysate;
    Lane 2: MHCDRa knockout Jurkat cell lysate;
    Predicted MW: 29kDa
    Observed MW: 25kDa;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Western Blot; Sample: Human Raji cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Western Blot; Sample: Human Jurkat cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Western Blot; Sample: Mouse Spleen lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Western Blot; Sample: Mouse Liver lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human MHCDRa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human HLA-DR Polyclonal Antibody (Catalog # PAC166Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA)

The antibody is a rabbit polyclonal antibody raised against HLA-DRA. It has been selected for its ability to recognize HLA-DRA in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA)

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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