Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
- Product No.PAA698Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA698Mu01-Recombinant Macrophage Migration Inhibitory Factor (MIF)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 227
For more details, please contact local distributors! US$ 324
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For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse MIF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse MIF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse MIF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant MIF, Mouse.
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Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Mastadenoma lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse MIF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
The antibody is a rabbit polyclonal antibody raised against MIF. It has been selected for its ability to recognize MIF in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| APA698Mu01 | Active Macrophage Migration Inhibitory Factor (MIF) | Cell culture; Activity Assays. |
| RPA698Mu02 | Recombinant Macrophage Migration Inhibitory Factor (MIF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA698Mu01 | Recombinant Macrophage Migration Inhibitory Factor (MIF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA698Mu01 | Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) | WB; IHC; ICC; IP. |
| PAA698Mu02 | Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) | WB; IHC; ICC; IP. |
| SEA698Mu | ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA698Mu | CLIA Kit for Macrophage Migration Inhibitory Factor (MIF) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA698Mu | Multiplex Assay Kit for Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |