Polyclonal Antibody to Cytokeratin 8 (CK8)
K8; CARD2; KRT8; CYK8; K2C8; KO; Keratin 8; Keratin, type II cytoskeletal 8
- Product No.PAC025Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC025Hu01-Recombinant Cytokeratin 8 (CK8)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species Moren/a
- ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 105
For more details, please contact local distributors!US$ 245
For more details, please contact local distributors! US$ 350
For more details, please contact local distributors! US$ 875
For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CK8 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant CK8, Human.
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Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: KRT8 knockout Hela cell lysate;
Predicted MW: 54,57kDa
Observed MW: 57kDa
Primary Ab: 2µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate
Primary Ab: 0.05µg/ml Rabbit Anti-Human CK8 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CK8 Polyclonal Antibody (Catalog PAC025Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Cytokeratin 8 (CK8)
The antibody is a rabbit polyclonal antibody raised against CK8. It has been selected for its ability to recognize CK8 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Cytokeratin 8 (CK8)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Cytokeratin 8 (CK8)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Cytokeratin 8 (CK8)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPC025Hu01 | Recombinant Cytokeratin 8 (CK8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC025Hu02 | Polyclonal Antibody to Cytokeratin 8 (CK8) | WB; IHC; ICC; IP. |
| PAC025Hu01 | Polyclonal Antibody to Cytokeratin 8 (CK8) | WB,IHC,ICC/IF |
| LAC025Hu81 | FITC-Linked Polyclonal Antibody to Cytokeratin 8 (CK8) | WB; IHC; ICC; IF. |
| LAC025Hu71 | Biotin-Linked Polyclonal Antibody to Cytokeratin 8 (CK8) | WB; IHC; ICC. |
| MAC025Hu21 | Monoclonal Antibody to Cytokeratin 8 (CK8) | WB; IHC; ICC; IP. |
| MAC025Hu23 | Monoclonal Antibody to Cytokeratin 8 (CK8) | WB; IHC; ICC; IP. |
| MAC025Hu22 | Monoclonal Antibody to Cytokeratin 8 (CK8) | WB; IHC; ICC; IP. |
| SEC025Hu | ELISA Kit for Cytokeratin 8 (CK8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC025Hu | Multiplex Assay Kit for Cytokeratin 8 (CK8) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |