Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- Product No.PAA693Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA693Mu01-Recombinant Heat Shock Protein 27 (Hsp27)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 227
For more details, please contact local distributors! US$ 324
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For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPB1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPB1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant HSPB1, Mouse.
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Western Blot; Sample: Mouse Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse HSPB1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Skeletal muscle lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse HSPB1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse HSPB1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)
The antibody is a rabbit polyclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA693Mu02 | Recombinant Heat Shock Protein 27 (Hsp27) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA693Mu01 | Recombinant Heat Shock Protein 27 (Hsp27) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA693Mu01 | Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) | WB; IHC; ICC; IP. |
| PAA693Mu02 | Polyclonal Antibody to Heat Shock Protein 27 (Hsp27) | WB; IHC; ICC; IP. |
| SEA693Mu | ELISA Kit for Heat Shock Protein 27 (Hsp27) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA693Mu | Multiplex Assay Kit for Heat Shock Protein 27 (Hsp27) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA693Mu01 | ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27) | Main materials for "Do It(ELISA Kit) Yourself" |