Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
Intermediate Filament Protein
- Product No.PAA068Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA068Hu01-Recombinant Glial Fibrillary Acidic Protein (GFAP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.45mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 95
For more details, please contact local distributors!US$ 221
For more details, please contact local distributors! US$ 316
For more details, please contact local distributors! US$ 790
For more details, please contact local distributors! US$ 3160
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Rat Cerebrum Tissue Primary Ab: 10µg/ml Rabbit Anti-Human GFAP Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Rat Cerebellum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GFAP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GFAP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GFAP, Human.
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Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: U87MG cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human GFAP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: U87MG cell lysate; Lane3: HBMEC cell lysate;
Primary Ab: 0.05µg/ml Rabbit Anti-Human GFAP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human U87MG cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human GFAP Polyclonal Antibody (Catalog PAA068Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
The antibody is a rabbit polyclonal antibody raised against GFAP. It has been selected for its ability to recognize GFAP in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
For flow cytometry, the suggested use of this reagent is 5-20 µg/mL per 10^6 cells in 100 µl volume;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service