Polyclonal Antibody to Forkhead Box Protein O1 (FOXO1)
FOX-O1; FKH1; FKHR; FOXO1A; Forkhead Homolog In Rhabdomyosarcoma
- Product No.PAA764Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA764Hu01-Recombinant Forkhead Box Protein O1 (FOXO1)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.25mg/ml
- Organism Species MoreMus musculus (Mouse)
- ApplicationsWB; IHC; IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 95
For more details, please contact local distributors!US$ 221
For more details, please contact local distributors! US$ 316
For more details, please contact local distributors! US$ 790
For more details, please contact local distributors! US$ 3160
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Esophagus Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human FOXO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human FOXO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant FOXO1, Human.
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Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human FOXO1 Polyclonal Antibody (Catalog PAA764Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Forkhead Box Protein O1 (FOXO1)
The antibody is a rabbit polyclonal antibody raised against FOXO1. It has been selected for its ability to recognize FOXO1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Forkhead Box Protein O1 (FOXO1)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Forkhead Box Protein O1 (FOXO1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Forkhead Box Protein O1 (FOXO1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA764Hu01 | Recombinant Forkhead Box Protein O1 (FOXO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA764Hu01 | Polyclonal Antibody to Forkhead Box Protein O1 (FOXO1) | WB; IHC; IF |
| MAA764Hu22 | Monoclonal Antibody to Forkhead Box Protein O1 (FOXO1) | WB; IHC; ICC; IP. |
| SEA764Hu | ELISA Kit for Forkhead Box Protein O1 (FOXO1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA764Hu | Multiplex Assay Kit for Forkhead Box Protein O1 (FOXO1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |