Polyclonal Antibody to Enolase 1 (ENO1)

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

  • Polyclonal Antibody to Enolase 1 (ENO1) Packages (Simulation)
  • Polyclonal Antibody to Enolase 1 (ENO1) Packages (Simulation)
  • Polyclonal Antibody to Enolase 1 (ENO1) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) DAB staining on IHC-P;
    Samples: Mouse Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) DAB staining on IHC-P;
    Sample: Mouse Skeletal muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) DAB staining on IHC-P;
    Sample: Mouse Skeletal muscle Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Enolase 1 (ENO1) FITC staining on IF;
    Sample: MCF7 cell
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB449Mu01.jpg Figure. Western Blot; Sample: Recombinant ENO1, Mouse.
  • Polyclonal Antibody to Enolase 1 (ENO1) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: NNE knockout MCF7 cell lysate;
    Predicted MW: 36,47kDa
    Observed MW: 50kDa
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) Western Blot; Sample: Mouse Placenta lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) Western Blot; Sample: Rat Mammary gland lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) Western Blot; Sample: Mouse Colon lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Enolase 1 (ENO1) Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Enolase 1 (ENO1)

The antibody is a rabbit polyclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Enolase 1 (ENO1)

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Enolase 1 (ENO1)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Enolase 1 (ENO1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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