Polyclonal Antibody to Connexin 43 (CX43)
ODDD; GJAL; GJA1; GJ-A1; Gap junction 43 kDa heart protein; Gap Junction Protein Alpha 1; Oculodentodigital Dysplasia; Syndactyly Type III
- Product No.PAA277Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA277Ra01-Recombinant Connexin 43 (CX43)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Rat Cardiac Muscle Tissue; Primary Ab: 10μg/ml Rabbit Anti-Rat CX43 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat CX43 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant CX43, Rat.
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Western Blot; Samples: Lane1: Rat Heart lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Cerebellum lysate; Lane4: Mouse Cerebrum lysate; Lane5: Mouse Cerebellum lysate; Lane6: U87MG cell lysate; Lane7: SK-N-SH cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Rat CX43 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Hela cell lysate
Primary Ab: 1µg/ml Rabbit Anti-Rat CX43 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Connexin 43 (CX43)
The antibody is a rabbit polyclonal antibody raised against CX43. It has been selected for its ability to recognize CX43 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Connexin 43 (CX43)
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Connexin 43 (CX43)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Connexin 43 (CX43)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA277Ra01 | Recombinant Connexin 43 (CX43) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA277Ra01 | Polyclonal Antibody to Connexin 43 (CX43) | WB; IHC; ICC; IP. |
| MAA277Ra21 | Monoclonal Antibody to Connexin 43 (CX43) | WB; IHC; ICC; IP. |
| SEA277Ra | ELISA Kit for Connexin 43 (CX43) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA277Ra | Multiplex Assay Kit for Connexin 43 (CX43) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |