Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule
- Product No.PAA781Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA781Hu01-Recombinant Cluster Of Differentiation 276 (CD276)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species Moren/a
- ApplicationsWB,IHC,FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 95
For more details, please contact local distributors!US$ 221
For more details, please contact local distributors! US$ 316
For more details, please contact local distributors! US$ 790
For more details, please contact local distributors! US$ 3160
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Vector Red staining on IHC-P;
Samples: Human Placenta Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant CD276, Human.
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Western Blot; Sample: Lane1: 293T cell lysate; Lane2: PC3 cell lysate; Lane3: MCF7 cell lysate
Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: CD276 knockout 293T cell lysate;
Predicted MW: 57,53,33kd
Observed MW: 100kd
Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
ChoK1 transfected with human CD276（Stable transfection） was stained with rabbit Anti-human CD276 Polyclonal Antibody (Catalog # PAA781Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
The antibody is a rabbit polyclonal antibody raised against CD276. It has been selected for its ability to recognize CD276 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA781Hu01 | Active Cluster Of Differentiation 276 (CD276) | Cell culture; Activity Assays. |
| RPA781Hu01 | Recombinant Cluster Of Differentiation 276 (CD276) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA781Hu01 | Polyclonal Antibody to Cluster Of Differentiation 276 (CD276) | WB,IHC,FCM |
| FAA781Hu01 | Anti-Cluster Of Differentiation 276 (CD276) Polyclonal Antibody | Flow cytometry. |
| MAA781Hu22 | Monoclonal Antibody to Cluster Of Differentiation 276 (CD276) | WB |
| MAA781Hu21 | Monoclonal Antibody to Cluster Of Differentiation 276 (CD276) | WB; IHC; ICC; IP. |
| SEA781Hu | ELISA Kit for Cluster Of Differentiation 276 (CD276) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA781Hu | Multiplex Assay Kit for Cluster Of Differentiation 276 (CD276) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |