Polyclonal Antibody to Beclin 1 (BECN1)
ATG6; VPS30; Coiled-Coil,Myosin-Like BCL2 Interacting Protein; ATG6 Autophagy Related 6 Homolog
- Product No.PAJ557Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPJ557Hu01-Recombinant Beclin 1 (BECN1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep)
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 105
For more details, please contact local distributors!US$ 245
For more details, please contact local distributors! US$ 350
For more details, please contact local distributors! US$ 875
For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human BECN1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Pancreas Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human BECN1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant BECN1, Human.
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Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate; Lane4: Canine Cerebrum lysate; Lane5: Bovine Cerebrum lysate; Lane6: Caprine Cerebrum lysate; Lane7: T-47D cell lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Human BECN1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Figure. Western Blot; Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Brain Tissue.
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Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: BECN1 knockout MCF7 cell lysate;
Predicted MW: 52kDa
Observed MW: 60kDa
Primary Ab: 3µg/ml Rabbit Anti-Human BECN1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Ovine Cerebrum lysate; Lane2: Bovine Cerebrum lysate; Lane3: MCF7 cell lysate; Lane4: A431 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human BECN1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Beclin 1 (BECN1)
The antibody is a rabbit polyclonal antibody raised against BECN1. It has been selected for its ability to recognize BECN1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Beclin 1 (BECN1)
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Beclin 1 (BECN1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Beclin 1 (BECN1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPJ557Hu01 | Recombinant Beclin 1 (BECN1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ557Hu01 | Polyclonal Antibody to Beclin 1 (BECN1) | WB,IHC |
| MAJ557Hu21 | Monoclonal Antibody to Beclin 1 (BECN1) | WB; IHC; ICC; IP. |
| MAJ557Hu22 | Monoclonal Antibody to Beclin 1 (BECN1) | WB; IHC; ICC; IP. |
| MAJ557Hu23 | Monoclonal Antibody to Beclin 1 (BECN1) | WB; IHC; ICC; IP. |
| MAJ557Hu24 | Monoclonal Antibody to Beclin 1 (BECN1) | WB; IHC; ICC; IP. |
| MAJ557Hu25 | Monoclonal Antibody to Beclin 1 (BECN1) | WB; IHC; ICC; IP. |
| SEJ557Hu | ELISA Kit for Beclin 1 (BECN1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ557Hu | Multiplex Assay Kit for Beclin 1 (BECN1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |