Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)
FBA; ALDO-A; Fructose 1,6 Bisphosphate Aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase
- Product No.PAA642Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA642Hu01-Recombinant Aldolase A, Fructose Bisphosphate (ALDOA)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Rat Colon Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human ALDOA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human ALDOA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Skeletal muscle Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Sample: HepG2 cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Human ALDOA Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
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Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 2µg/mL Rabbit Anti-Human ALDOA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: Human Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Liver lysate; Lane3: Rat Cerebrum lysate; Lane4: Rat Small intestine lysate; Lane5: Rat Skeletal muscle lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected -
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human ALDOA Polyclonal Antibody (Catalog # PAA642Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)
The antibody is a rabbit polyclonal antibody raised against ALDOA. It has been selected for its ability to recognize ALDOA in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA642Hu01 | Recombinant Aldolase A, Fructose Bisphosphate (ALDOA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA642Hu01 | Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA) | WB; IHC; ICC; IP. |
| FAA642Hu01 | Anti-Aldolase A, Fructose Bisphosphate (ALDOA) Polyclonal Antibody | Flow cytometry. |
| MAA642Hu21 | Monoclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA) | WB; IHC; ICC; IP. |
| SEA642Hu | ELISA Kit for Aldolase A, Fructose Bisphosphate (ALDOA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA642Hu | Multiplex Assay Kit for Aldolase A, Fructose Bisphosphate (ALDOA) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |