Polyclonal Antibody to Actinin Alpha 2 (ACTN2)
Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein
- Product No.PAA376Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA376Mu01-Recombinant Actinin Alpha 2 (ACTN2)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 227
For more details, please contact local distributors! US$ 324
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For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse ACTN2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant ACTN2, Mouse.
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Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: ACTN2 knockout Hela cell lysate;
Predicted MW: 104kDa
Observed MW: 104kDa
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Skeletal muscle lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Actinin Alpha 2 (ACTN2)
The antibody is a rabbit polyclonal antibody raised against ACTN2. It has been selected for its ability to recognize ACTN2 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Actinin Alpha 2 (ACTN2)
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Actinin Alpha 2 (ACTN2)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Actinin Alpha 2 (ACTN2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA376Mu01 | Recombinant Actinin Alpha 2 (ACTN2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA376Mu01 | Polyclonal Antibody to Actinin Alpha 2 (ACTN2) | WB,IHC |
| LAA376Mu71 | Biotin-Linked Polyclonal Antibody to Actinin Alpha 2 (ACTN2) | WB; IHC; ICC. |
| LAA376Mu81 | FITC-Linked Polyclonal Antibody to Actinin Alpha 2 (ACTN2) | WB; IHC; ICC; IF. |