Polyclonal Antibody to Aconitase 1 (ACO1)
IREB1; IRP1; IREBP; Iron regulatory protein 1; Iron-responsive element-binding protein 1; Cytoplasmic aconitate hydratase; Citrate hydro-lyase; Ferritin repressor protein
- Product No.PAJ683Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPJ683Hu02-Recombinant Aconitase 1 (ACO1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; ICC; IHC-P; IHC-F; ELISA.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 105
For more details, please contact local distributors!US$ 245
For more details, please contact local distributors! US$ 350
For more details, please contact local distributors! US$ 875
For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human ACO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant ACO1, Human.
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Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human SiHa cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ACO1 Polyclonal Antibody (Catalog PAJ683Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Aconitase 1 (ACO1)
The antibody is a rabbit polyclonal antibody raised against ACO1. It has been selected for its ability to recognize ACO1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Aconitase 1 (ACO1)
Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Aconitase 1 (ACO1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Aconitase 1 (ACO1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPJ683Hu02 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu03 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu04 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu01 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ683Hu04 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; ICC; IHC-P; IHC-F; ELISA. |
| PAJ683Hu02 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; ICC; IHC-P; IHC-F; ELISA. |
| PAJ683Hu01 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; ICC; IHC-P; IHC-F; ELISA. |
| PAJ683Hu03 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; IHC; ICC; IP. |
| LAJ683Hu71 | Biotin-Linked Polyclonal Antibody to Aconitase 1 (ACO1) | WB; IHC; ICC. |
| SEJ683Hu | ELISA Kit for Aconitase 1 (ACO1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ683Hu | Multiplex Assay Kit for Aconitase 1 (ACO1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |