Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody
G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
- Product No.CAB932Mi22
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreHamster (Chinese hamster), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)
- ApplicationsLoading Control of WB
- Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 107
For more details, please contact local distributors!US$ 251
For more details, please contact local distributors! US$ 358
For more details, please contact local distributors! US$ 895
For more details, please contact local distributors! US$ 3580
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate Primary Ab: 1µg/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Porcine Cerebrum lysate; Lane4: Bovine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Equine Liver lysate; Lane7: Caprine Cerebrum lysate
Primary Ab: 3µg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot analysis of GAPDH in various cell lines
Lane1: DU145 whole cell lysate
Lane2: OS-RC-2 whole cell lysate
Lane3: T-47D whole cell lysate
Lane4: HEC-1B whole cell lysate
Lane5: HepG2 whole cell lysate
Lane6: HEK-293 whole cell lysate
Lysates/proteins at 20µg per lane.
Primary Ab: anti-GAPDH antibody (CAB932Mi22) at 0.1ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique.
Calculated MW: 36KD
Observed MW: 36KD
Exposure time: 1 min -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Liver lysate; Lane4: Cavia Kidney lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Liver lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Caprine Liver lysate; Lane10: Mouse Liver lysate; Lane11: Rat Liver lysate; Lane12: Rat Kidney lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody
The antibody is a mouse monoclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.
USAGE of the Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody
Enzyme-Linked Immune Sorbent Assay:
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| CAB932Mi01 | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Polyclonal Antibody | Loading Control of WB. |
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