Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

CD120B; P75; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR2; TNFR80; P75tnfr; Tumor necrosis factor receptor 2; p80 TNF-alpha receptor; Etanercept

  • Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Packages (Simulation)
  • Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Packages (Simulation)
  • Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Figure. Gene Sequencing (Extract)
  • APB504Hu01.png Figure. SDS-PAGE
  • Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) , also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Besides, Tumor Necrosis Factor Alpha (TNFa) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNFa. Briefly, TNFRSF1B were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNFa-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF1B and TNFa was shown in Figure 1, the EC50 was 0.0470~0.085 ug/ml.

USAGE of the Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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