Active Tumor Necrosis Factor Alpha (TNFa)

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

  • Active Tumor Necrosis Factor Alpha (TNFa) Packages (Simulation)
  • Active Tumor Necrosis Factor Alpha (TNFa) Packages (Simulation)
  • Active Tumor Necrosis Factor Alpha (TNFa) Gene sequencing
  • APA133Mu61.jpg SDS-PAGE
  • Active Tumor Necrosis Factor Alpha (TNFa) Figure. Western Blot; Sample: APA133Mu61; Antibody: PAA133Mu06
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Tumor Necrosis Factor Alpha (TNFa)

TNF-a, being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .<br/>Cell necrosis after incubation with TNF-a (10ng/mL) for 72h was shown in Figure 1.

The production of IL-1β after incubation with TNF-a (10ng/mL) for 8h is shown in Table 1.

USAGE of the Active Tumor Necrosis Factor Alpha (TNFa)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Tumor Necrosis Factor Alpha (TNFa)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Tumor Necrosis Factor Alpha (TNFa)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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