Active Transforming Growth Factor Beta 1 (TGFb1)

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

  • Active Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • Active Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • Active Transforming Growth Factor Beta 1 (TGFb1) Gene sequencing
  • APA124Hu01.jpg SDS-PAGE
  • Active Transforming Growth Factor Beta 1 (TGFb1) Figure. Western Blot; Sample: Recombinant TGFb1, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Transforming Growth Factor Beta 1 (TGFb1)

TGF-β1 (Transforming growth factor beta 1) is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF beta 1 has been shown to interact with TGF beta receptor 1, Decorin, LTBP1 and so on. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human TGF-β1 with recombinant human LTBP1. Briefly, TGF-β1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LTBP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LTBP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-β1 with LTBP1 was shown in Figure 1 and this effect was in a dose dependent manner.

USAGE of the Active Transforming Growth Factor Beta 1 (TGFb1)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Transforming Growth Factor Beta 1 (TGFb1)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Transforming Growth Factor Beta 1 (TGFb1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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