Active Regenerating Islet Derived Protein 3 Beta (REG3b)

PAP1; Pancreatitis-Associated Protein 1

  • Active Regenerating Islet Derived Protein 3 Beta (REG3b) Packages (Simulation)
  • Active Regenerating Islet Derived Protein 3 Beta (REG3b) Packages (Simulation)
  • APK541Mu01.jpg SDS-PAGE
  • Active Regenerating Islet Derived Protein 3 Beta (REG3b) Figure. Western Blot; Sample: Recombinant REG3b, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Regenerating Islet Derived Protein 3 Beta (REG3b)

Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of REG3b. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of REG3b was shown in Figure 2. It was obvious that REG3b significantly promoted cell proliferation of SK-N-SH cells. The ED50 for this effect is typically3.685-84.37ng/mL.

USAGE of the Active Regenerating Islet Derived Protein 3 Beta (REG3b)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Regenerating Islet Derived Protein 3 Beta (REG3b)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Regenerating Islet Derived Protein 3 Beta (REG3b)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
APK541Mu01 Active Regenerating Islet Derived Protein 3 Beta (REG3b) Cell culture; Activity Assays.
RPK541Mu01 Recombinant Regenerating Islet Derived Protein 3 Beta (REG3b) Positive Control; Immunogen; SDS-PAGE; WB.
RPK541Mu02 Recombinant Regenerating Islet Derived Protein 3 Beta (REG3b) Positive Control; Immunogen; SDS-PAGE; WB.
PAK541Mu01 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Beta (REG3b) WB; IHC; ICC; IP.
PAK541Mu02 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Beta (REG3b) WB; IHC; ICC; IP.
SEK541Mu ELISA Kit for Regenerating Islet Derived Protein 3 Beta (REG3b) Enzyme-linked immunosorbent assay for Antigen Detection.
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