Active Nitric Oxide Synthase 2, Inducible (NOS2)
NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2
- Product No.APA837Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point7.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. SDS-PAGE
- Figure. Western Blot
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Nitric Oxide Synthase 2, Inducible (NOS2)
Figure. The binding activity of NOS2 with UCHL5.
Nitric oxide synthase 2, inducible (NOS2) is a member of Nitric oxide synthases (NOSs) family. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of NOS2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NOS2 and recombinant mouse UCHL5. Briefly, NOS2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NOS2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NOS2 and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Nitric Oxide Synthase 2, Inducible (NOS2)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Nitric Oxide Synthase 2, Inducible (NOS2)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Nitric Oxide Synthase 2, Inducible (NOS2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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