Active Myelin Basic Protein (MBP)

Myelin A1 protein; Myelin membrane encephalitogenic protein

  • Active Myelin Basic Protein (MBP) Packages (Simulation)
  • Active Myelin Basic Protein (MBP) Packages (Simulation)
  • APA539Hu61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Myelin Basic Protein (MBP)

Myelin basic protein (MBP), a 18.5-kDa encephalitogenic peptide, is the second most abundant in central nervous system and responsible for adhesion of the cytosolic surfaces of multilayered compact myelin, MBP is biased expressed in cerebellum adult and cortex adult. It can interact with a number of polyanionic proteins including actin, tubulin, Ca(2 )-calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. Calmodulin Like Protein 3 (CALML3) is a kind of Ca(2 )-calmodulin, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MBP and recombinant human CALML3. Briefly, biotin-linked MBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALML3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinan human MBP and recombinan human CALML3 was shown in Figure 1, the EC50 for this effect is 7.4 ug/mL.

USAGE of the Active Myelin Basic Protein (MBP)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Myelin Basic Protein (MBP)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Myelin Basic Protein (MBP)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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