Active Microfibrillar Associated Protein 2 (MFAP2)
MAGP1; Microfibril-associated glycoprotein 1
- Product No.APC618Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.5
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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ACTIVITY TEST of the Active Microfibrillar Associated Protein 2 (MFAP2)
Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Microfibrillar Associated Protein 2 (MFAP2)
Reconstitute in ddH2O to a concentration ≤0.1mg/mL. Do not vortex.
STORAGE of the Active Microfibrillar Associated Protein 2 (MFAP2)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Microfibrillar Associated Protein 2 (MFAP2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPC618Hu01 | Recombinant Microfibrillar Associated Protein 2 (MFAP2) | Positive Control; Immunogen; SDS-PAGE; WB. |
APC618Hu01 | Active Microfibrillar Associated Protein 2 (MFAP2) | Cell culture; Activity Assays. |
PAC618Hu01 | Polyclonal Antibody to Microfibrillar Associated Protein 2 (MFAP2) | WB; IHC; ICC; IP. |