Active Matrix Metalloproteinase 9 (MMP9)

GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

  • Active Matrix Metalloproteinase 9 (MMP9) Packages (Simulation)
  • Active Matrix Metalloproteinase 9 (MMP9) Packages (Simulation)
  • APA553Po01.png Figure. SDS-PAGE
  • Active Matrix Metalloproteinase 9 (MMP9) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Matrix Metalloproteinase 9 (MMP9)

Figure. Hydrolysis of gelatin by MMP9.
Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000ng, 500ng, 100ng, 10ng, 1ng, 0.1ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control.
Result: Gelatin hydrolysis by recombinant pig MMP9 (10-70kd) was shown in figure 1.

USAGE of the Active Matrix Metalloproteinase 9 (MMP9)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Matrix Metalloproteinase 9 (MMP9)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Matrix Metalloproteinase 9 (MMP9)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Sus scrofa; Porcine (Pig) Applications (RESEARCH USE ONLY!)
APA553Po01 Active Matrix Metalloproteinase 9 (MMP9) Cell culture; Activity Assays.
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