Active Interleukin 17D (IL17D)
Interleukin-27
- Product No.APD346Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.1
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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SDS-PAGE
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Figure. Western Blot; Sample: Recombinant IL17D, Human.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 17D (IL17D)
IL17D (Interleukin-17D) belongs to the IL17 (interleukin 17) family, which includes six members (IL-17, IL-17B through IL-17F). IL17D is reported to induce the expression of IL6, CXCL8/IL8, and CSF2/GM-CSF from endothelial cells. IL17 family members share some similarities in AA sequence and structure, thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17D with recombinant human IL17RB. Briefly, IL17D were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RB-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17D pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17D with IL17RB was shown in Figure 1 and this effect was in a dose dependent manner.
USAGE of the Active Interleukin 17D (IL17D)
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 17D (IL17D)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 17D (IL17D)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPD346Hu01 | Recombinant Interleukin 17D (IL17D) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD346Hu01 | Active Interleukin 17D (IL17D) | Cell culture; Activity Assays. |
| PAD346Hu01 | Polyclonal Antibody to Interleukin 17D (IL17D) | WB; IHC; ICC; IP. |
| MAD346Hu21 | Monoclonal Antibody to Interleukin 17D (IL17D) | WB; IHC; ICC; IP. |
| SED346Hu | ELISA Kit for Interleukin 17D (IL17D) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD346Hu | Multiplex Assay Kit for Interleukin 17D (IL17D) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |