Active Interleukin 17C (IL17C)
- Product No.APD347Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point8.2
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors!US$ 600
For more details, please contact local distributors! US$ 1200
For more details, please contact local distributors! US$ 3600
For more details, please contact local distributors! US$ 9000
For more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Figure. SDS-PAGE
- Figure. Western Blot; Sample: Recombinant IL17C, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 17C (IL17C)
IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
Figure. The binding activity of IL17C with IL17RA.
USAGE of the Active Interleukin 17C (IL17C)
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 17C (IL17C)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 17C (IL17C)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
BCA Protein Quantification Kit
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|RPD347Hu01||Recombinant Interleukin 17C (IL17C)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APD347Hu01||Active Interleukin 17C (IL17C)||Cell culture; Activity Assays.|
|PAD347Hu01||Polyclonal Antibody to Interleukin 17C (IL17C)||WB; IHC; ICC; IP.|
|MAD347Hu21||Monoclonal Antibody to Interleukin 17C (IL17C)||WB; IHC; ICC; IP.|
|SED347Hu||ELISA Kit for Interleukin 17C (IL17C)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCD347Hu||CLIA Kit for Interleukin 17C (IL17C)||Chemiluminescent immunoassay for Antigen Detection.|