Active Interleukin 17C (IL17C)

Cytokine CX2

  • Active Interleukin 17C (IL17C) Packages (Simulation)
  • Active Interleukin 17C (IL17C) Packages (Simulation)
  • APD347Hu01.jpg Figure. SDS-PAGE
  • Active Interleukin 17C (IL17C) Figure. Western Blot; Sample: Recombinant IL17C, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Interleukin 17C (IL17C)

IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
Figure. The binding activity of IL17C with IL17RA.

USAGE of the Active Interleukin 17C (IL17C)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Interleukin 17C (IL17C)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Interleukin 17C (IL17C)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPD347Hu01 Recombinant Interleukin 17C (IL17C) Positive Control; Immunogen; SDS-PAGE; WB.
APD347Hu01 Active Interleukin 17C (IL17C) Cell culture; Activity Assays.
PAD347Hu01 Polyclonal Antibody to Interleukin 17C (IL17C) WB; IHC; ICC; IP.
MAD347Hu21 Monoclonal Antibody to Interleukin 17C (IL17C) WB; IHC; ICC; IP.
SED347Hu ELISA Kit for Interleukin 17C (IL17C) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD347Hu CLIA Kit for Interleukin 17C (IL17C) Chemiluminescent immunoassay for Antigen Detection.