Active Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- Product No.APA049Gu01
- Organism SpeciesCavia (Guinea pig ) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors!US$ 875
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For more details, please contact local distributors! US$ 13125
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- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant IFNg, Cavia.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interferon Gamma (IFNg)
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. It has been reported that IFN-γ promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages as an important activator. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 264.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 264.7 cells were incubated in DMEM with IFN-γ (10ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA.
Effect of IFN-γ on morphological change of Raw 246.7 cells was shown in Figure 1.
Effect of IFN-γ on the expression of iNOS was shown in Table 1.
USAGE of the Active Interferon Gamma (IFNg)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interferon Gamma (IFNg)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interferon Gamma (IFNg)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Molecular Mass Marker for Protein
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|Catalog No.||Organism species: Cavia (Guinea pig )||Applications (RESEARCH USE ONLY!)|
|APA049Gu01||Active Interferon Gamma (IFNg)||Cell culture; Activity Assays.|
|RPA049Gu01||Recombinant Interferon Gamma (IFNg)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA049Gu01||Polyclonal Antibody to Interferon Gamma (IFNg)||WB; IHC; ICC; IP.|
|LAA049Gu91||HRP-Linked Polyclonal Antibody to Interferon Gamma (IFNg)||WB; IHC; ICC.|
|LAA049Gu71||Biotin-Linked Polyclonal Antibody to Interferon Gamma (IFNg)||WB; IHC; ICC.|
|MAA049Gu21||Monoclonal Antibody to Interferon Gamma (IFNg)||WB; IHC; ICC; IP.|
|SEA049Gu||ELISA Kit for Interferon Gamma (IFNg)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA049Gu||CLIA Kit for Interferon Gamma (IFNg)||Chemiluminescent immunoassay for Antigen Detection.|