Active High Mobility Group Protein 1 (HMGB1)

HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

  • Active High Mobility Group Protein 1 (HMGB1) Packages (Simulation)
  • Active High Mobility Group Protein 1 (HMGB1) Packages (Simulation)
  • Active High Mobility Group Protein 1 (HMGB1) Figure. Gene Sequencing (Extract)
  • APA399Hu61.png Figure. SDS-PAGE
  • Active High Mobility Group Protein 1 (HMGB1) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active High Mobility Group Protein 1 (HMGB1)

Figure 1. The binding activity of HMG1 with TP53.<br/>High Mobility Group Protein 1 (HMG1), also known as high mobility group box 1 proteinbelongs to high mobility group and contains HMG-box domain. HMG1 is one of the most important chromatin proteins. This nuclear protein organizes the DNA and regulates transcription. It supports transcription of many genes in interactions with many transcription factors. HMGB1 is secreted by immune cells (like macrophages, monocytes and dendritic cells) through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. Besides, Tumor Protein p53 (TP53) has been identified as an interactor of HMG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HMG1 and recombinant human TP53. Briefly, HMG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TP53-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HMG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of HMG1 and TP53 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active High Mobility Group Protein 1 (HMGB1)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active High Mobility Group Protein 1 (HMGB1)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active High Mobility Group Protein 1 (HMGB1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APA399Hu02 Active High Mobility Group Protein 1 (HMGB1) Cell culture; Activity Assays.
EPA399Hu62 Eukaryotic High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
EPA399Hu63 Eukaryotic High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
EPA399Hu61 Eukaryotic High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA399Hu02 Recombinant High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
APA399Hu61 Active High Mobility Group Protein 1 (HMGB1) Cell culture; Activity Assays.
RPA399Hu01 Recombinant High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA399Hu06 Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
PAA399Hu01 Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
PAA399Hu02 Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
LAA399Hu71 Biotin-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC.
LAA399Hu81 FITC-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IF.
MAA399Hu22 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
MAA399Hu21 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
SEA399Hu ELISA Kit for High Mobility Group Protein 1 (HMGB1) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA399Hu Instant ELISA Kit for High Mobility Group Protein 1 (HMGB1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA399Hu CLIA Kit for High Mobility Group Protein 1 (HMGB1) Chemiluminescent immunoassay for Antigen Detection.
KSA399Hu01 ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMGB1) Main materials for "Do It(ELISA Kit) Yourself"
PGA399Hu01 Primer Pair for High Mobility Group Protein 1 (HMGB1) PCR
GUESTBOOK