Active Granzyme K (GZMK)
GZM-K; TRYP2; PRSS; Serine Protease,Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2
- Product No.APB209Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.5
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$
For more details, please contact local distributors!US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Granzyme K (GZMK)
Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Human granzyme K is synthesized as a precursor (264 residues) with a signal peptide (residues 1 24), a propeptide (residues 25-26) and a mature chain (residues 27-264). The purified recombinant human Granzyme K consists of residues 27 to 264 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 µL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme K is >220 pmol/min/µg.
USAGE of the Active Granzyme K (GZMK)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Granzyme K (GZMK)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Granzyme K (GZMK)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB209Hu01 | Recombinant Granzyme K (GZMK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB209Hu01 | Active Granzyme K (GZMK) | Cell culture; Activity Assays. |
| PAB209Hu01 | Polyclonal Antibody to Granzyme K (GZMK) | WB; IHC; ICC; IP. |
| LAB209Hu81 | FITC-Linked Polyclonal Antibody to Granzyme K (GZMK) | WB; IHC; ICC; IF. |
| LAB209Hu71 | Biotin-Linked Polyclonal Antibody to Granzyme K (GZMK) | WB; IHC; ICC. |
| MAB209Hu21 | Monoclonal Antibody to Granzyme K (GZMK) | WB; IHC; ICC; IP. |
| MAB209Hu22 | Monoclonal Antibody to Granzyme K (GZMK) | WB; IHC; ICC; IP. |
| SEB209Hu | ELISA Kit for Granzyme K (GZMK) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB209Hu | Multiplex Assay Kit for Granzyme K (GZMK) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |