Active Endonuclease G, Mitochondrial (ENDOG)
- Product No.APC457Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.5
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant ENDOG, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Endonuclease G, Mitochondrial (ENDOG)
ENDOG (Endonuclease G) is a mitochondrial enzyme that cleaves DNA at double-stranded (DG)n. (DC)n and at single-stranded (DC)n tracts. This protein also has ribonuclease (RNase) and RNase H activities. It has been reported that EndoG forms complexes with some other proteins, including HSP70 (Heat shock 70 kDa protein). Besides, mouse HSPA1A (Heat shock 70 kDa protein 1A) shares similarities with rat HSP1A1 in amino acid sequence with the identity of 98.44%. Thus, a binding ELISA assay was conducted to detect the association of recombinant rat ENDOG with recombinant mouse HSPA1A. Briefly, ENDOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP1A1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ENDOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ENDOG with HSP1A1 was shown in Figure 1 and this effect was in a dose dependent manner.
USAGE of the Active Endonuclease G, Mitochondrial (ENDOG)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Endonuclease G, Mitochondrial (ENDOG)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Endonuclease G, Mitochondrial (ENDOG)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Rattus norvegicus (Rat)||Applications (RESEARCH USE ONLY!)|
|RPC457Ra01||Recombinant Endonuclease G, Mitochondrial (ENDOG)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC457Ra01||Active Endonuclease G, Mitochondrial (ENDOG)||Cell culture; Activity Assays.|
|PAC457Ra01||Polyclonal Antibody to Endonuclease G, Mitochondrial (ENDOG)||WB; IHC; ICC; IP.|
|MAC457Ra21||Monoclonal Antibody to Endonuclease G, Mitochondrial (ENDOG)||WB; IHC; ICC; IP.|
|SEC457Ra||ELISA Kit for Endonuclease G, Mitochondrial (ENDOG)||Enzyme-linked immunosorbent assay for Antigen Detection.|