Active Coagulation Factor VII (F7)
FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa
- Product No.APB874Ra02
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant F7, Rat.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Coagulation Factor VII (F7)
The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating FVII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as an interactor of FVII, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FVII and recombinant rat TF. Briefly, FVII were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FVII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of FVII and TF was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Coagulation Factor VII (F7)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Coagulation Factor VII (F7)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Coagulation Factor VII (F7)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Rattus norvegicus (Rat)||Applications (RESEARCH USE ONLY!)|
|RPB874Ra01||Recombinant Coagulation Factor VII (F7)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB874Ra02||Recombinant Coagulation Factor VII (F7)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APB874Ra02||Active Coagulation Factor VII (F7)||Cell culture; Activity Assays.|
|PAB874Ra01||Polyclonal Antibody to Coagulation Factor VII (F7)||WB; IHC; ICC; IP.|
|PAB874Ra02||Polyclonal Antibody to Coagulation Factor VII (F7)||WB; IHC; ICC; IP.|
|LAB874Ra71||Biotin-Linked Polyclonal Antibody to Coagulation Factor VII (F7)||WB; IHC; ICC.|
|SEB874Ra||ELISA Kit for Coagulation Factor VII (F7)||Enzyme-linked immunosorbent assay for Antigen Detection.|