ELISA Kit DIY Materials for Nephrin (NPHN)
NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor
- Product No.KSA937Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleurine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.113ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Download n/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Nephrin (NPHN)
The Abs in the kit have high sensitivity and excellent specificity for detection of Nephrin (NPHN). No significant cross-reactivity or interference between Nephrin (NPHN) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Nephrin (NPHN)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Nephrin (NPHN)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| KSA937Mu01 | ELISA Kit DIY Materials for Nephrin (NPHN) | Main materials for "Do It (ELISA Kit) Yourself". |