ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)
BP4; HT29-IGFBP; IBP4
- Product No.KSA055Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.129ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Download n/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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FOB
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)
The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 4 (IGFBP4).No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)