Active Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- Product No.APA047Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$ 308
For more details, please contact local distributors!US$ 770
For more details, please contact local distributors! US$ 1540
For more details, please contact local distributors! US$ 4620
For more details, please contact local distributors! US$ 11550
For more details, please contact local distributors!
ACTIVITY TEST of the Active Hepatocyte Growth Factor (HGF)
Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing. Besides, Matrix Metalloproteinase 9 (MMP9) has been identified as an interactor of HGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HGF and recombinant mouse MMP9. Briefly, HGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MMP9 -coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti- HGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human HGF and recombinant mouse MMP9 was shown in Figure 1, the EC50 for this effect is 0.35 ug/mL.
USAGE of the Active Hepatocyte Growth Factor (HGF)
Reconstitute in ddH2O to a concentration less than or equal to 0.1mg/mL. Do not vortex.
STORAGE of the Active Hepatocyte Growth Factor (HGF)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Hepatocyte Growth Factor (HGF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A